Volume 3,Issue 2
咖啡豆多肽的制备及其生物活性表征
通本研究以咖啡豆为原料，采用酶解技术制备咖啡豆多肽，表征了结构并探究其对人皮肤成纤维细胞（HDF-α）氧化损伤的保护作用及胶原代谢调控机制。采用GPC测定相对分子质量分布，LC-MS/MS鉴定氨基酸序列，建立H₂O₂联合IL-1β诱导的HDF-α细胞氧化损伤模型，并通过RT-qPCR检测COL-Ⅰ、COL-Ⅲ、MMP-1、MMP-3 中mRNA相关基因的表达。结果表明，所制备咖啡豆多肽重均相对分子质量（Mw）为4751 Da，多分散系数1.391，相对分子质量分布集中；其主要多肽序列富含疏水性及碱性氨基酸，三肽占比为60%。细胞实验表明，在12.5~50 mg/kg浓度范围内，咖啡豆多肽显著促进HDF-α细胞增殖（P < 0.001），并对氧化损伤具显著保护作用（P < 0.05~0.01）。机制研究发现，咖啡豆多肽可显著上调COL-Ⅰ/Ⅲ的mRNA表达（P < 0.01），同时显著抑制MMP-1/3 的mRNA表达（P < 0.01），有效调控胶原代谢平衡。该咖啡豆多肽具有抗氧化和抗光老化活性，可作为功能性原料应用于化妆品领域。
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