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Volume 3,Issue 2

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25 June 2026

V型胶原蛋白及A型重组V型人源化胶原蛋白的研究进展与应用展望

宪瑶 孟1 艺 张1 传秀 陈1
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1 山西锦波生物医药股份有限公司, 中国
CDCST 2026 , 3(2), 140–146; https://doi.org/10.61369/CDCST.2026020023
© 2026 by the Author(s). Licensee Art and Technology, USA. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution -Noncommercial 4.0 International License (CC BY-NC 4.0) ( https://creativecommons.org/licenses/by-nc/4.0/ )
Abstract

V型胶原蛋白(COLV)是细胞外基质中的关键调节性纤维胶原,随着对COLV结构-功能研究的深入及合成生物学的发展,A型重组V型人源化胶原蛋白的制备成为可能。本文系统综述了天然COLV的分子结构特征及皮肤生物学功能,并探讨了A型重组V型人源化胶原蛋白的生物合成机制及多元化应用前景。目前A型重组V型人源化胶原蛋白与天然COLV在结构完整性和功能等同性方面仍存在差异,其应用仍需进一步验证。

Keywords
V型胶原蛋白
A 型重组人源化
生物合成
生物学功能
护肤应用
References

[1]Jordi Bella,David J. S. Hulmes. Fibrillar Collagens[M]. Cham:Springer International Publishing, 2017: 457-490.
[2]Ki M Mak,Chien Yi M Png,Danielle J Lee. Type V Collagen inHealth, Disease, and Fibrosis[J]. Anatomical Record (hoboken, N.j. :2007), 2016, 299(5): 613-29.
[3]Birk D E. Type V collagen: heterotypic type I/V collagen interactionsin the regulation of fibril assembly.
[J]. Micron (oxford, England : 1993),2001(3): 223-37.
[4]Xinzhi Zhou,Zhaocheng Weng,Chen Zhao, et al. CollagenBiosynthesis to Engineered Biomaterials: Molecular Design, SyntheticStrategy, and Biomedical Application[J]. Scie Publishing Limited, 2025,3(3): 10013-10013.
[5]Jinyuan Hu,Junhui Li,Jennifer Jiang, et al. Design of syntheticcollagens that assemble into supramolecular banded fibers as afunctional biomaterial testbed[J]. Springer Science and Business MediaLlc, 2022, 13(1): 6761.
[6]Richard J. Wenstrup,Jane B. Florer,Eric W. Brunskill, et al. TypeV Collagen Controls the Initiation of Collagen Fibril Assembly[J].Elsevier Bv, 2004, 279(51): 53331-53337.
[7]Konstantinos Andrikopoulos,Xin Liu,Douglas R. Keene, et al.Targeted mutation in the col5a2 gene reveals a regulatory role for typeV collagen during matrix assembly[J]. Springer Science and BusinessMedia Llc, 1995, 9(1): 31-36.
[8]Sheng Wen,Ruimin Ren,Hanhao Yuan, et al. Col5a3 Likely PromotesAdipogenesis of 3T3-L1 Through Oxidative Phosphorylation[J]. MdpiAg, 2025, 16(2): 165.
[9]Guorui Huang,Gaoxiang Ge,Dingyan Wang, et al. α3(V) Collagenis critical for glucose homeostasis in mice due to effects in pancreaticislets and peripheral tissues[J]. American Society for ClinicalInvestigation, 2011, 121(2): 769-783.
[10]C Niyibizi,P P Fietzek,M van der Rest. Human placenta type Vcollagens. Evidence for the existence of an alpha 1(V) alpha 2(V) alpha3(V) collagen molecule.
[J]. Elsevier Bv, 1984, 259(22): 14170-14174.
[11]Christelle Bonod-Bidaud,Muriel Roulet,Uwe Hansen, et al. In vivoevidence for a bridging role of a collagen V subtype at the epidermisdermisinterface.
[J]. Elsevier Bv, 2012, 132(7): 1841-1849.
[12]Greenspan Daniel S.. Biosynthetic Processing of CollagenMolecules[M]. Berlin, Heidelberg: Springer Berlin Heidelberg, 2005:149-183.
[13]Matthew D. Shoulders,Ronald T. Raines. Collagen Structure andStability[J]. Annual Reviews, 2009, 78(1): 929-958.
[14]Jean-Marie Bourhis,Natacha Mariano,Yuguang Zhao, et al.Structural basis of fibrillar collagen trimerization and related geneticdisorders[J]. Springer Science and Business Media Llc, 2012, 19(10):1031-1036.
[15]D E Birk,J M Fitch,J P Babiarz, et al. Collagen type I and type Vare present in the same fibril in the avian corneal stroma.
[J]. RockefellerUniversity Press, 1988, 106(3): 999-1008.
[16]T F Linsenmayer,E Gibney,F Igoe, et al. Type V collagen:molecular structure and fibrillar organization of the chicken alpha 1(V)NH2-terminal domain, a putative regulator of corneal fibrillogenesis.
[J].Rockefeller University Press, 1993, 121(5): 1181-1189.
[17]Gary J. Fisher,Sewon Kang,James Varani, et al. Mechanisms ofPhotoaging and Chronological Skin Aging[J]. American MedicalAssociation (ama), 2002, 138(11):1462–1470.
[18]Gary J. Fisher,James Varani,John J. Voorhees. Looking Older[J].American Medical Association (ama), 2008, 144(5): 666–672..
[19]Fransiska Malfait,Richard J. Wenstrup,Anne De Paepe. Clinical andgenetic aspects of Ehlers-Danlos syndrome, classic type[J]. Elsevier Bv,2010, 12(10): 597-605.
[20]Richard A. F. Clark. Overview and General Considerations ofWound Repair[M]. Boston, Ma: Springer Us, 1998: 3-33.
[21]Yoko Hashimoto,Tien-Yu Shieh,Hisashi Aoyama, et al. Isolation and characterization of type V collagen from human post-burngranulation tissues.
[J]. Elsevier Bv, 1986, 87(4): 540-543.
[22]Steven R. Beanes,Catherine Dang,Chia Soo, et al. Skin repair andscar formation: the central role of TGF-β[J]. Cambridge UniversityPress (cup), 2003, 5(8): 1-22.
[23]Cédric Zeltz,Joseph Orgel,Donald Gullberg. Molecular compositionand function of integrin-based collagen glues—IntroducingCOLINBRIs[J]. Elsevier Bv, 2014, 1840(8): 2533-2548.
[24]Joseph P.R.O. Orgel,Rama S. Madhurapantula. A structuralprospective for collagen receptors such as DDR and their binding of thecollagen fibril[J]. Elsevier Bv, 2019, 1866(11): 118478.
[25]Birgit Leitinger,Erhard Hohenester. Mammalian collagenreceptors[J]. Elsevier Bv, 2007, 26(3): 146-155.
[26]Chuan-Xiu Chen,Ya-Yao Zhang,Jingbo Yang, et al. An overviewof progress in the application of recombinant collagen in cosmetics[J].Elsevier Bv, 2024, 1(4): 100059.
[27]Su Liu,Yanmei Li,Meng Wang, et al. Efficient coexpression ofrecombinant human fusion collagen with prolyl 4 ‐hydroxylase fromBacillus anthracis in Escherichia coli[J]. Biotechnology and AppliedBiochemistry, 2023, 70(2): 761-772.
[28]Wang Jing,Xiang Zhi-Xiang,Luan Meng-Fan,et al. High-level secretoryexpression of recombinant type III human-like collagen α1 inPichia pastoris via multilevel systematic optimization.
[J]. InternationalJournal of Biological Macromolecules, 2025: 144270.
[29]Chuan Wang,Xiaolei Guo,Mingtao Fan, et al. Production of recombinanthuman type I collagen homotrimers in CHO cells and theirphysicochemical and functional properties[J]. Journal of Biotechnology,2024, 395: 149-160.
[30]Stein Hanan,Wilensky Michal,Tsafrir Yehuda,et al.Production ofbioactive, post-translationally modified, heterotrimeric, human recombinanttype-I collagen in transgenic tobacco.
[J]. Biomacromolecules,2009, (9): 2640-5.
[31]Lamberg A, Helaakoski T, Myllyharju J, et al. Characterization ofHuman Type III Collagen Expressed in a Baculovirus System Journal ofBiological Chemistry, 1996, 271: 11988-11995.
[32]Chung-Jr Huang, Henry Lin, Xiaoming Yang. Industrial productionof recombinant therapeutics in Escherichia coli and its recent advancements[J]. Oxford University Press (oup), 2012, 39(3): 383-399.
[33]Christine Unsöld,William N. Pappano,Yasutada Imamura, et al.Biosynthetic Processing of the Pro-α1(V)2Pro-α2(V) Collagen Heterotrimerby Bone Morphogenetic Protein-1 and Furin-like ProproteinConvertases[J]. Elsevier Bv, 2002, 277(7): 5596-5602.
[34]Christoph Rutschmann,Stephan Baumann,Jürg Cabalzar, et al. Recombinantexpression of hydroxylated human collagen in Escherichiacoli[J]. Springer Science and Business Media Llc, 2014, 98(10): 4445-4455.
[35]Eesha Khare,Chi-Hua Yu,Constancio Gonzalez Obeso, et al. Discoveringdesign principles of collagen molecular stability using a geneticalgorithm, deep learning, and experimental validation[J]. Proceedingsof the National Academy of Sciences, 2022, 119(40).
[36]J. Myllyharju,M. Nokelainen,A. Vuorela, et al. Expression of recombinanthuman collagens in the yeast Pichia pastoris[J]. PortlandPress Ltd., 2000, 28(3): A55-A55.
[37]Zhou H, Li W, Pan L, et al. Human extracellular matrix (ECM)-likecollagen and its bioactivity[J]. Regenerative Biomaterials, 2024,rbae008.
[38]F Ruggiero,J.-Y Exposito,P Bournat, et al. Triple helix assemblyand processing of human collagen produced in transgenic tobaccoplants[J]. Wiley, 2000, 469(1): 132-136.
[39]Hsiu-Chuan Li,Chuan-Chuan Huang,Sung-Fang Chen, et al. Assemblyof homotrimeric type XXI minicollagen by coexpression ofprolyl 4-hydroxylase in stably transfected Drosophila melanogaster S2cells[J]. Elsevier Bv, 2005, 336(2): 375-385.
[40]杨霞,杨立,王云兵, 等.一种生物合成人体结构性材料的制备方法:202210849498[P][2026-05-26].
[41]Zhu Y.,Yang X.,Sun F.. Structure of a triple-helix region of humancollagen type V[Z]: Worldwide Protein Data Bank, 2023.
[42]Isaac Wong Kai Jie,Kar Wai Alvin Lee,Song Eun Yoon, et al. Advancementsin Clinical Utilization of Recombinant Human Collagen:An Extensive Review[J]. Life (basel, Switzerland), 2025, 15(4): 582.
[43]Huang Can,Li Zhihui,Wu Shuang,et al. Bridging the gap inpre-market immunological evaluation: a progressive strategy for recombinantcollagen medical devices[J], 2025, 8(1).
[44]Zhang Zhiyu,Yang Wenqing,Yang Jingbo,et al. Evaluation of thesafety and functional effects of recombinant humanized type III collagenin food toxicology[J]. Frontiers in Medicine, 2026, 13: 1765276.
[45]杨霞,陈传秀,孟宪瑶, 等.一种重组V型人源化胶原蛋白及其应用:CN202511079735.2[P].CN120988100A[2026-05-26].
[46]Florian Labarrade,Imane Garcia,Isabelle Imbert. Collagen type Valpha 3 chain is involved in human skin basement membrane physiologyand MMP ‐9 regulation[J]. Wiley, 2025, 47(5): 743-751.
[47]Roel G M Breuls,Darinka D Klumpers,Vincent Everts, et al. Collagentype V modulates fibroblast behavior dependent on substrate stiffness[J]. Biochemical and Biophysical Research Communications, 2009,380(2): 425-9.
[48]Mei Sun,Shoujun Chen,Sheila M. Adams, et al. Collagen V is adominant regulator of collagen fibrillogenesis: dysfunctional regulationof structure and function in a corneal-stroma-specificCol5a1-null mousemodel[J]. The Company of Biologists, 2011, 124(23): 4096-4105.
[49]Prashant Chandrasekaran,Bryan Kwok,Biao Han, et al. Type Vcollagen regulates the structure and biomechanics of TMJ condylar cartilage:A fibrous-hyaline hybrid[J]. Elsevier Bv, 2021, 102: 1-19.
[50]Melanie L. Dart,Ewa Jankowska-Gan,Guorui Huang, et al. Interleukin-17–Dependent Autoimmunity to Collagen Type V in Atherosclerosis[J]. Ovid Technologies (wolters Kluwer Health), 2010, 107(9): 1106-1116.

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