Volume 3,Issue 4
营养生长阶段的大豆镍胁迫响应：生长与生理特征
为探究营养生长阶段镍胁迫对大豆的生理毒性效应，本研究以中豆7901大豆为材料，设置土壤镍浓度0、30、60、120、240 mg/kg，测定分枝期植株生物量、叶片氧化损伤及抗氧化酶活性。结果显示：随镍浓度升高，大豆根茎叶生长受显著抑制，根在30mg/kg时重量略有上升然后随浓度进一步升高而持续下降，茎叶随浓度升高持续下降；叶片H₂O₂ 与MDA含量持续上升，氧化应激与膜脂过氧化加剧；SOD、CAT活性先升后降，30 mg/kg达峰值，高浓度下酶系统受损。研究表明，大豆营养生长期对镍胁迫的耐受临界浓度为30 ～ 60 mg/kg，240 mg/kg超出耐受阈值；SOD‑CAT级联清除是大豆响应镍胁迫的核心抗氧化机制。本研究为耐镍大豆品种选育与农田镍污染风险评估提供理论依据。
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