Volume 4,Issue 5
GADD45G在不明原因复发性流产患者蜕膜组织中的异常表达的研究
【目的】探索GADD45G基因在复发性流产患者蜕膜组织中的异常表达、基因富集分析、免疫分析及生物学功能。并进行临床样本RT-PCR验证其差异性。【方法】基于GEO数据库检索并筛选复发性流产相关基因表达矩阵。对合并数据集进行归一化，利用机器算法联合ROC曲线筛选出关键基因GADD45G，对关键基因进行功能富集分析、信号通路、免疫浸润、药物靶点预测分析了解其相关功能。随后RT-PCR验证GADD45G在复发性流产患者蜕膜组织中的表达水平。【结果】GADD45G基因在RSA患者蜕膜组织中异常高表达，且富集分析显示其可能多基因联合参与影响卵母细胞发育异常、细胞周期紊乱、钙离子转运异常及免疫调控失衡密切相关参与复发性流产的病理生理过程。GADD45G基因与RSA患者蜕膜组织免疫细胞浸润密切相关。RT-QPCR实验结果证实复发性流产患者蜕膜组织的GADD45G基因的表达明显高于人工流产组（P ＜ 0.05）。【结论】GADD45G是复发性流产患者蜕膜组织中异常高表达的关键基因，GADD45G基因与RSA患者蜕膜组织免疫细胞浸润相关，GADD45G可能作为复发性流产的潜在生物标记物和治疗靶点。
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