Volume 4,Issue 9
消渴固肾方干预2型糖尿病模型大鼠血清代谢重塑的非靶向代谢组学研究
目的：基于LC-MS 非靶向血清代谢组学，解析消渴固肾方对高脂饮食联合链脲佐菌素诱导2型糖尿病大鼠血清代谢重塑的作用。方法：设正常、模型、消渴固肾方低/ 中/ 高剂量、阳性对照及QC 组，行LC-MS 采集、差异分析、PCA/PLS-DA、KEGG 富集及WGCNA 分析。结果：纳入34个样本，获690个代谢特征，映射率88.41%。模型组较正常组筛得109个差异代谢物（63个上调、46个下调），富集于胆汁分泌、鞘脂代谢及甾体激素生物合成通路。低、中、高剂量组分别筛得75、61、55个差异代谢物，PC(16:0/20:5) 在三组均呈反向调节。WGCNA 识别5个模块，筛选出SM(d18:0/16:1)、氢化可的松、胆酸等候选关键代谢物。结论：消渴固肾方系统性重塑T2DM 大鼠血清代谢组，涉及胆汁酸、鞘脂代谢及甾体激素合成通路，为后续机制验证提供候选靶点。
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