Volume 4,Issue 9
六味地黄丸/RXRa/PI3K-AKT 轴向调控前列腺癌的作用机制
六味地黄丸（LWDHP）在临床实践中展现出对抗前列腺癌的潜在疗效，然而其背后的作用机制仍未阐明。本研究整合网络药理预测、生物信息分析及实验验证，旨在揭示该方剂发挥抗癌功效的分子基础。通过筛选LWDHP 所含活性组分，获取药物靶点与疾病相关靶标的交集；实施网络拓扑结构解析与功能富集评估；并借助蛋白质印迹法检测关键蛋白的表达水平。最终鉴定出69种具有生物活性的化学实体，以及186个核心作用基因；其中槲皮素被确认为主要活性成分，重要靶点涵盖RXRa、PTGS2与HSP90AA1；核心基因富集于PI3K-Akt 和TNF 通路。实验证实，LWDHP 下调前列腺癌细胞RXRa 表达并改变AKT 磷酸化水平。LWDHP 通过“多成分- 多靶点- 多通路”协同模式，尤其调节RXRa 介导的PI3K-Akt 信号轴，发挥抗前列腺癌效应。
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